Journal: Translational Psychiatry
Article Title: Double-hit of MIA and Nod2 deficiency induces sex-specific offspring behavioral abnormalities through placental dysregulation
doi: 10.1038/s41398-025-03747-z
Figure Lengend Snippet: A Hematoxylin and eosin staining of placental tissue. Left: Overall structure (Scale bar: 1000 μm); Right: Magnification of sinusoids (Scale bar: 100 μm). B Immunofluorescence for cluster of differentiation 31 ( CD31 ) (red) and 4′,6-diamidino-2-phenylindole (DAPI) (blue). From left to right: Overview (Scale bar: 1000 μm), CD31 channel, DAPI channel, and merged image (Scale bar: 100 μm). C Immunofluorescence for cytokeratin 7 ( CK7 ) (red) and DAPI (blue). Panels arranged as in B. D Comparison of placental efficiency (fetal-to-placental weight ratio) between groups. E Ratio of labyrinth zone to total area (labyrinth + junction zone). F Quantification of CD31 fluorescence intensity. G Quantification of CK7 fluorescence intensity. Sample size: n = 12 per group (male: female = 1:1). Data expressed as mean ± SEM; Student’s t -test or the non-parametric Wilcoxon rank-sum test.
Article Snippet: Subsequently, the sections were incubated separately with primary antibodies against cytokeratin 7 ( CK7 ) (1:400, 17513-1-AP; Proteintech) and cluster of differentiation 31 ( CD31 ) (1:400, 28083-1-AP; Proteintech), each diluted in PBS, overnight at 4 °C.
Techniques: Staining, Immunofluorescence, Comparison, Fluorescence